Earlier Friday, head coach Doug Marrone said Spiller will play Sunday against the Miami Dolphins.
Defensive tackle Kyle Williams, who missed Friday's practice due to an illness, is also probable, along with cornerback Ron Brooks, who could play for the first time since fracturing his foot in the season opener.
Here is the full Bills injury report:
QB EJ Manuel (knee)
CB Ron Brooks (foot)
RB C.J. Spiller (ankle)
LB Manny Lawson (hamstring)
DE Mario Williams (hip)
DT Kyle Williams (illness/Achilles)
WR Stevie Johnson (lower back)
QB Thad Lewis (foot)