Johnson's mother died unexpectedly last Saturday, but he played in Sunday's win over the Jacksonville Jaguars.
"I don't believe that he will be available for our game on Sunday," coach Doug Marrone said. "Our thoughts and our prayers are with Stevie and his family."
The status of safety Aaron Williams, who left Sunday's game with an injury to his ribs, is up in the air.
"I think Aaron is going to be 50-50," Marrone said. I can't really say right now either way, so we're going to be monitoring that situation and going through that treatment process."
Here is the Bills' full injury report from Wednesday:
DID NOT PARTICIPATE
S Aaron Williams (ribs)
DT Kyle Williams (Achilles)
RB Fred Jackson (ribs)
WR Stevie Johnson (personal)
LIMITED IN PRACTICE
CB Brandon Smith (ankle)
DT Marcell Dareus (ankle)
QB EJ Manuel (knee)