"If I can go, I'll go," Spiller said. "If I can't, I can't."
ESPN NFL Insider Adam Schefter reported Monday that Spiller will play.
Spiller walked through the locker room with a heavy limp Tuesday evening after practice. He said there is "not much swelling" in his ankle, which he injured in last Sunday's win over the Baltimore Ravens.
"It felt good. To be honest, I didn't think I would be able to jog today. So that was an encouraging sign," Spiller said. "Tomorrow will be a huge day for us, to see what the reaction will be."
Fred Jackson, who sprained his left MCL on Sunday, was limited in practice. He wore a brace over his injured knee.
The Bills are expected to give Tashard Choice more carries Thursday night, and coach Doug Marrone said Tuesday that the team may consider signing running back Ronnie Wingo off the practice squad.