Buckles, who was averaging 8.2 points and a team-leading 7.5 rebounds, will miss the rivalry game with Kentucky tomorrow at the KFC Yum! Center, where he was to be the guy to have defended Wildcats freshman sensation Terrence Jones.
"It affects [the rotation] signifcantly," Pitino said, adding that Stephan Van Treese would have to step up.
"It doesn't affect us mentally because we're used to all these injuries we've been having."
Not that Kentucky will feel sorry for Louisville, but coach John Calipari feels his counterpart has been doing "a great job" with the personnel he has.
"Typically what (Coach Pitino) does is he gets his team, he figures them out, they play to their strengths, and that's what they are doing," he said. "They are playing to their strengths. They do it very well, and they are doing it as well as anybody in the country."