Carlos Ortiz was arraigned Tuesday in Fall River Superior Court on a murder charge in connection with the June 2013 death of Odin Lloyd. He is being held without bail and a pre-trial conference is set for Sept. 24.
Ortiz is from Hernandez's hometown of Bristol, Connecticut. Ortiz also pleaded not guilty earlier to an accessory charge.
Ortiz's lawyer has called the murder indictment "totally ridiculous."
Hernandez has also pleaded not guilty to murder in the death of Lloyd, who was dating Hernandez's fiancee's sister. He will be also arraigned Wednesday in Boston on two counts of first degree murder and other charges, in connection with a July 2012 double shooting in Boston
A third man is also facing murder charge in connection with Lloyd's death.
The Associated Press contributed to this report.