He skated lightly before the rest of his teammates took to the ice for the morning skate but head coach Joel Quenneville told reporters Monday morning he would not play.
Without Ladd, the Blackhawks beat the Flyers 2-1 to take a 2-0 series lead.
Ladd was injured in the fourth and final game of the Western Conference finals against San Jose.
His replacement in the lineup, Tomas Kopecky, scored the game-winning goal in Game 1, a 6-5 Chicago victory.
While Ladd remained out for Chicago, Philadelphia forward Daniel Carcillo returned to the Flyers lineup for Game 2. Carcillo was a healthy scratch for three games.
"You battle all year with these guys and you want to be in at this time of year," Carcillo said Monday morning. "It was hard sitting out those three games. I've got a lot of pent-up energy you'll probably see released tonight."
James van Riemsdyk exited the Flyers lineup to make room for Carcillo.
Scott Burnside covers the NHL for ESPN.com.