His backup, redshirt freshman Skyler Mornhinweg, will make his second career start, first at Ben Hill Griffin Stadium.
The Gators (4-6, 3-5 in the SEC) play host to FCS Georgia Southern (6-4, 4-4 Southern) at 2 p.m. ET.
Murphy, who sprained the AC joint in his throwing shoulder in the LSU game on Oct. 12 and aggravated it in the Gators' Nov. 9 loss to Vanderbilt, led UF to a 2-4 record in six starts after starter Jeff Driskel was lost for the season with a broken bone in his lower right leg. Murphy passed for 1,216 yards with six touchdowns and five interceptions.
With the inexperienced Mornhinweg making his first start last week in a 19-14 loss at South Carolina, the Gators went with a very conservative game plan. He was limited to five passing attempts until there was less than seven minutes remaining in the game.
But Florida coaches promised an expanded workload for Mornhinweg in his next action.
"We need to expect more of Skyler now," offensive coordinator Brent Pease said Tuesday. "I think he can handle it. I've got confidence in him that he can."
Florida also announced on Saturday it would play without starting defensive end Jonathan Bullard, who aggravated a hamstring injury during practice this week.
The Gators have also suspended true freshman wide receiver Demarcus Robinson for the remainder of the season (two games) for a violation of team rules.