Frierson pleaded not guilty at his arraignment Monday. He is alleged to have punched two people early Sunday on a street near the Illinois campus before fleeing the scene.
"As I have always done, I review each situation on an individual basis and make sure I have all the information," Illinois coach Ron Zook said in a prepared statement. "After a full review, I think it's in Evan's best interest to concentrate on his academics and his legal issues, and he no longer remains a part of our football program."
Frierson has appeared in nine games this season and recorded six tackles. His next court date is Dec. 21.
Police spokeswoman Rene Dunn says the 20-year-old Frierson was arrested just after midnight Sunday in Champaign after an argument and fight. She said Frierson is accused of punching two men who were standing in a crosswalk as cars tried to drive through. One of the men required medical attention.
Police said Frierson told the man to leave, but the man tried to spit on Frierson, prompting Frierson to react.
Adam Rittenberg covers Big Ten football for ESPN.com. Information from The Associated Press was used in this report.