Five players were discharged from the hospital on Friday and six more left the facility on Saturday. The remaining two were released Sunday.
"Getting all 13 student-athletes healthy and out of the hospital has been priority number one all along, so I'm very happy that they all are now back home and resuming their lives," Iowa coach Kirk Ferentz said in a school statement.
The players were hospitalized Monday with rhabdomyolysis, a stress-induced syndrome that can damage cells and cause kidney problems. The muscle disorder developed following a series of grueling offseason workouts.
University leaders announced Thursday that they've ordered an investigation into how the players wound up in the hospital, with Iowa Board of Regents President David Miles and school president Sally Mason saying the hospitalizations were "a cause for grave concern."
They agreed the university will have 90 days to complete an investigation analyzing events leading up to their hospitalization, and that the review will involve independent medical experts.
Information from The Associated Press contributed to this report.