Coach Jim Caldwell still has not said why Wayne was out.
The five-time Pro Bowler hadn't practiced since last Wednesday and skipped Friday night's preseason game against Washington. Indy lost 16-3.
On Monday, Caldwell said he expected Wayne back within 24 hours. He made it back onto the field Tuesday.
Wayne has been a vital cog in the Colts' high-scoring offense for years, first as the complimentary receiver to Marvin Harrison, then as Peyton Manning's No. 1 option. Last year, Wayne had a career-high 111 receptions for 1,355 yards and six touchdowns.
His return comes as Manning missed yet another practice while he rehabs from offseason surgery to repair a nerve in his neck.