Pelini told reporters after practice Wednesday that Rome's "personal goals and personal perception of where he should be on this football team doesn't match the team goals."
Rome's departure comes after the Cornhuskers' defensive line played poorly in a loss at UCLA on Saturday. Defensive coaches said early in the week that personnel changes were being considered at several positions.
Rome, a sophomore from Columbia, Mo., had four tackles in the first two games.
Pelini also announced that backup receiver Tyler Wullenwaber injured his knee in practice Tuesday and would miss the rest of the season.