Coach Bob Huggins announced Holton's suspension in a statement Thursday night. The statement didn't specify the reason for the suspension.
Holton has averaged 9.7 points and 7.5 rebounds this season and has started every game for the ninth-ranked Mountaineers (17-3). West Virginia plays at Florida (13-7) on Saturday in the Big 12/SEC Challenge.
In a 70-55 win over Kansas State on Tuesday night, Holton had 14 points and 11 rebounds, his second double-double over a three-game stretch.
His suspension could slow West Virginia's surprising run in the Big 12 race. The Mountaineers are tied for first place with No. 1 Oklahoma and No. 17 Baylor at 6-2.