Rolen was placed on the DL on Aug. 10 with left shoulder tightness. He is expected to work out with the team for a couple days before returning to the starting lineup.
"I'm going to have him in the lineup pretty much everyday," Blue Jays manager Cito Gaston said.
Rolen was hitting .252 with seven homers and 38 RBIs in 88 games. He also missed the first 23 games of the season after hurting his finger late in spring training.
Also, Gaston said reliever Jeremy Accardo will not pitch again this season due to a right forearm injury. The right-hander, on the disabled list since May 10, will be examined by Dr. James Andrews.
Accardo went 4-for-6 in save situations this season.