Chamberlain, pitching for Double-A Trenton in a camp game against Triple-A Scranton/Wikes-Barre, retired 16 in a row -- including five strikeouts -- after allowing two hits and a walk to start the game.
The Yankees' No. 5 starter faced additional batters after three outs were recorded -- two in the third and three during the fifth -- to reach his scheduled pitch count of 75.
"It took me just a little bit to get going," Chamberlain said. "I wanted to work on fastball command and my changeup, and I think I did a great job with those two. I think it was one of my most productive days as far as consistency. It was good."
Chamberlain flew to Florida on Saturday, one day after watching the Yankees' first game -- an exhibition contest with the Chicago Cubs -- at the new Yankee Stadium. He is scheduled to make his first regular-season start next Sunday at Kansas City.
"I'm excited to get going," he said.