He went 0-for-3 and played six innings in the field in an 11-3 loss to the Tampa Bay Rays on Tuesday night. He had a nice play behind the bag to throw out a runner at first.
Twins starter Vance Worley allowed 11 hits and seven earned runs in the loss. His spring ERA rose to 13.50, and he didn't get through the third inning.
Florimon had played a total of eight innings on the minor league side, then had his scheduled debut Monday rained out in Sarasota.
He underwent an appendectomy Feb. 17 after experiencing stomach pain. Because the procedure was to be done via laparoscopy, Florimon was expected to be out only a couple of weeks before resuming baseball activities.
Manager Ron Gardenhire told MLB.com at the time that he expected Florimon to be ready for Opening Day.
"[Florimon's] our shortstop," Gardenhire said. "[Jason] Bartlett is here to try and make the club, but Florimon is our shortstop."
Florimon, 27, hit .221 with nine home runs and 44 RBIs in 134 games with the Twins last season.
The Associated Press contributed to this report.