Reyes went 1-for-5 in the leadoff spot, including an RBI single in the second inning.
Reyes hadn't played since straining his left hamstring during his first at-bat of the season on March 31 against Tampa Bay. Blue Jays manager John Gibbons said Friday that Reyes will play every day.
The switch-hitting Reyes initially injured his hamstring during spring training. He played in two exhibitions before the opener, but after hitting a sinking liner to center in the first inning, he pulled up while running to first.
Reyes had been rehabbing his leg in Florida. He batted .296 last season for Toronto, but missed 66 games with a sprained left ankle.
The Blue Jays placed designated hitter Adam Lind on the 15-day DL after Friday's game because of a sore back.