Arcia was hurt after leading off the sixth inning with a double. When Trevor Plouffe whiffed on his bunt attempt, the Brewers caught Arcia with too big a lead off second base. Arcia rolled his right ankle when he tried to plant his foot and retreat, and he was picked off as he dove back into the bag.
Arcia walked gingerly to the dugout, not putting his full weight on his right leg, and was replaced in the field by Chris Parmelee.
Earlier in the night, Arcia hit a grand slam, his second home run in two games and the first slam by the Twins all season.
The burly 23-year-old gave the Twins a 4-0 lead in the third inning when he clobbered a 2-2 slider onto the open concourse behind the right-field seats. Arcia responded to his curtain call, emerging from the dugout, flipping off his helmet to reveal his faux-hawk haircut and thrusting both arms in the air to acknowledge the crowd.
Arcia is batting .366 in his past 10 games and .286 on the season.
The Associated Press contributed to this report.