Tulowitzki appeared to suffer the injury while running out an infield ground out in the bottom of the sixth inning. He returned to the dugout limping slightly and was replaced at short the next inning by Marco Scutaro, who slid over from second base. DJ LeMahieu came off the bench to replace Scutaro at second.
Tulowitzki is scheduled to be re-evaluated on Thursday.
"We don't know anything more than just that. We just have to wait and see to what extent," Rockies manager Jim Tracy said.
Carlos Gonzalez said he just wants to see Tulowitzki get healthy as soon as he can.
"I just want him to be healthy and get right and we all believe he's going to be even better," Gonzalez said. "He's showing signs that he's going to contribute a lot to this team, and I know he's going to do a lot of damage and help the team when he gets back."
Information from The Associated Press was used in this report.