Holliday was hit by an 89-mph fastball from Brewers starter Randy Wolf near his left knee in the first inning. After spending several minutes with the team's trainer, Holliday initially remained in the game. But Cardinals manager Mike Matheny took him out in the bottom of the second, when Jon Jay came in as a defensive replacement. Holliday's injury was described as a left thigh contusion.
Holliday had a game-tying two-run single in the ninth inning of Monday's game, which the Cardinals went on to win 3-2.
Information from The Associated Press was used in this report.