Wade described the pain as "just a little uncomfortable." He said he will have an MRI on the knee on Saturday.
"I gave it a couple days to see if it would go away," Wade said. "It didn't, so I just want to go back and get another, second opinion. But it's nothing I'm overly concerned about. It's just some soreness I haven't felt in a while, so I just want to make sure everything's good."
Wade's injury left the Heat with nine healthy players Friday night.
"Obviously it's never a good time," Wade said, "but if you say there's a bad time, this is a bad time."
Guard Gerald Green moved into the starting lineup for Wade.
The Associated Press contributed to this report.