Harden sprained his right ankle in a Feb. 12 road victory at Golden State and did not play the following game. The only other game he has missed this season involved a right ankle sprain that caused him to miss a Dec. 10 meeting against San Antonio.
Coach Kevin McHale said Harden had a shot a few weeks ago and kept playing through the injury.
"It's one of those things that you hope will get better soon because needless to say, we need him," McHale said.
Harden, who played 26 minutes and scored seven points in a 103-94 road loss to Memphis on Friday night, ranks fifth in the league with 26.0 points per game. That was just the second time this season where Harden failed to score in double figures.
"It's been bothering him for a little while, so he's going to be out tonight," McHale said.