Wade did not play in the second half of Miami's game against New Jersey on Tuesday after turning the ankle, though insisted afterward he could have returned if necessary. He sprained the same ankle and missed six games earlier this season.
Heat coach Erik Spoelstra said Wade tested the ankle Wednesday afternoon and experienced no problems.
"We made sure he's ready to go," Spoelstra said.
Meanwhile, the Hawks arrived in Miami at less than full strength.
Atlanta guard Joe Johnson was ruled out for the Hawks, marking the sixth game he's missed out of Atlanta's past seven -- as well as the All-Star game -- because of left knee tendinitis. Johnson did some shooting on Monday and had a pregame workout in Indiana on Tuesday, but it's still unknown when he will return to the lineup.
"I'm hoping he's close," Hawks coach Larry Drew said. "I know he's been working out every day. He says it's feeling better."
Tracy McGrady, who has been dealing with a left knee strain, was held out of the Atlanta lineup. McGrady played nearly 20 minutes Tuesday in Atlanta's win at Indiana, but Drew said he didn't want to subject McGrady to the demands of back-to-back games.
Reserve guard Willie Green also remained sidelined for Atlanta while recovering from back spasms. The Hawks have been dealing with the absences of centers Al Horford (left pectoral muscle tear) and Jason Collins (left elbow) for several weeks.
Wade aggravated the ankle on an awkward play against the Nets.
He appeared to step on the back of New Jersey reserve Jordan Williams' foot while chasing him down court with 1:25 left in the first half on Tuesday. Wade tumbled to the court, got up after a moment then walked without an obvious limp to Miami's offensive end when the Heat took possession.
Wade went to the bench and was quickly approached by Heat trainer Jay Sabol, but showed no signs of pain and received no treatment before Sabol returned to his seat a few seconds later. Wade was with his teammates on the bench for the second half against the Nets.