Pierce had to be helped off the field after getting stopped for a 2-yard on a second-and-5 at the Temple 12 midway through the third quarter. He was later carted from the sideline to the locker room.
A Temple spokeswoman said the team didn't give out injury updates during a game.
Pierce, who was being touted by the school as a Heisman Trophy candidate, had run for two first-quarter touchdowns. The sophomore rushed for 169 yards and three touchdowns last week in a win over Connecticut.