Jacobs, who rushed for 106 yards on 22 carries in the Giants' loss to the Chicago Bears in their last game 11 days ago, did not play because of a hamstring injury, a source said.
It also is possible that fullback John Conner could get a carry or two, although his role is more likely to be as a blocking back and possibly in pass protection.
Jacobs missed practice last Monday with the hamstring injury, but the team said it was just being cautious and he was back on the practice field Thursday.
But when Jacobs missed practice Saturday, it was because of an unforeseen setback, and the team listed him as questionable to play. The two days since have not improved the situation sufficiently.
Giants coach Tom Coughlin said Thursday that Hillis already knows the offense because of his time earlier this year in Tampa Bay with offensive coordinator and former Giants assistant Mike Sullivan.