The tackle reportedly occurred late in the third quarter, following a 36-yard run by Baltimore running back Ricky Williams in the Ravens' 35-7 victory.
"It definitely wasn't on purpose," Polamalu told the Pittsburgh Tribune-Review. "I've never intentionally tried to horse-collar anybody."
Polamalu's teammate, cornerback Ike Taylor, was also fined $15,000. Taylor was fined for unnecessary roughness.
Specifically, Taylor was fined for unnecessarily head-butting an opponent on a run play.
Ravens linebacker Brendon Ayanbadejo, like Polamalu, was fined $15,000 for a horse-collar tackle committed on a special teams play.
The NFL confirmed the fines on Friday. The league did not identify the plays but both probably involved Redskins tight end Fred Davis.
Rolle was fined $20,000 for spearing an opponent with his helmet. Rolle was flagged for an unnecessary roughness call against Davis with roughly eight minutes left in the Redskins' 28-14 win. The third-down penalty kept Washington's drive alive and it scored an insurance touchdown.
Phillips was fined $10,000 because he unnecessarily struck an opponent in the head and neck area. The only play in question involving Phillips happened late in the third quarter on a tackle of Davis. A penalty was not called.
Phillips indicated in the locker room on Friday that he planned to appeal the fine.
Chung got flagged for a personal foul with 11:29 to play in the first quarter when he tackled Dolphins running back Reggie Bush by the back of the helmet.
Information from ESPNBoston's Mike Reiss and The Associated Press was used in this report.