Dixon, a fourth-year player whose season high for carries is 70 as a rookie in 2010, posted his thoughts about the 49ers facing their NFC West rivals on his Twitter account Wednesday night.
He tweeted: "Extra weight on the racks all week getting less sleep preparing for these She-Hawks I love hostile environments Imma feel right at home..."
Dixon deleted the tweet Thursday morning, but not before it had been noticed and reported by several media outlets.
Seahawks linebacker K.J. Wright, a college teammate of Dixon's at Mississippi State, responded to Dixon's tweet on Wednesday night. He also later deleted his tweet.
In a reply to Dixon, he wrote: "lol the she hawks!! I'll be sure relay the message to the fellas.. Its gone be a long night for you and the forty whiners."
The 49ers held off the Seahawks last season to win the NFC West title for the second straight year, but San Francisco was blown out the last time it played at CenturyLink Field.
Dixon had just two carries for 8 yards in that game. He didn't have a carry in the 49ers' Week 1 victory over the Green Bay Packers but did have a 30-yard kickoff return.
This is the second straight week Dixon, 24, has made headlines. Last week he said he turned down a $24,000 offer from teammate Nnamdi Asomugha for his No. 24, which the veteran cornerback wanted to wear in his first season with the 49ers.
ESPN.com Seahawks reporter Terry Blount contributed to this report.