Manning played one possession in the preseason opener against the Chicago Bears on Thursday and got intercepted near the end zone.
In his first appearance since the Pro Bowl after the 2010 season while with Indianapolis, Manning completed 4 of 7 passes for 44 yards.
After a short run by Willis McGahee, Manning found Eric Decker on the left for 10 yards. A 19-yarder to Decker put the ball at the 14. But on third down at the 12, Manning was picked off when Denver's Brandon Stokley deflected a pass and Major Wright dived to intercept it.