ROTTERDAM, Netherlands -- Top-seeded Rafael Nadal became the fourth straight seeded player to lose Thursday in the second round of the ABN Amro.
The Spaniard lost to Andreas Seppi of Italy 3-6, 6-3, 6-4.
The world's No. 2 had been the last remaining seeded player in the draw. Second-seeded Nikolay Davydenko, third-seeded David Ferrer of Spain and fifth-seeded Tomas Berdych of the Czech Republic lost earlier Thursday.
It is only the third time since 2000 that there are no seeded players in the quarterfinals of an ATP tournament. It last happened in 2006 in Nottingham, England, and before that in 2002 in Buenos Aires, Argentina.
Nadal broke Seppi twice to take the opening set, but the Italian rebounded to win the second. Down two breaks in the third set, Nadal saved two match points in the eighth game and then broke on his fourth break point.
The three-time French Open champion held serve at love in the next game, but Seppi closed out the match by holding at love on his 24th birthday.
Nadal challenged the call on his final forehand, but replays confirmed it was long and Seppi raised both fists in triumph.
Llodra converting all three break-point opportunities while Davydenko only capitalized on one from eight. Davydenko received treatment for a shoulder injury in the second set.
Ferrer smashed two rackets in his loss to Zverev, who reached his third quarterfinal. Zverev broke twice in each set while only dropping serve once, in the second set.
"I expected to be sitting at home in Hamburg tonight," Zverev said. "This is the biggest win of my career."
Zverev will play Karlovic, who had 17 aces against Berdych.