U.S. captain Jim Courier announced the team in a statement Wednesday from Melbourne, where he is working at the Australian Open.
The match against Brazil, the first tie at home for the U.S. team since the 2011 quarterfinals, will be played on an indoor hard court in Jacksonville, Fla.
Isner pulled out of the Australian Open with a right knee injury, while Querrey lost in the third round.
The Bryan brothers, the Olympic doubles gold medalists, won their quarterfinal match Wednesday at the Australian Open.
The winner of the match will face either Serbia or Belgium in the quarterfinals from April 5-7.